Assemblies-Of-Putative-SARS-CoV2-Spike-Encoding-MRNA-Sequences-For-Vaccines-BNT-162b2-And-MRNA-1273

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Assemblies-of-putative-SARS-CoV2-spike-encoding-mRNA-sequences-for-vaccines-BNT-162b2-and-mRNA-1273 is an academic research tool that provides experimental sequence information for COVID-19 mRNA vaccines. It offers data for analyzing the BNT-162b2 and mRNA-1273 vaccine sequences.

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Pricing

Free · Open Source

Free tier

Yes

API

Skill level

Technical

About

What is Assemblies-of-putative-SARS-CoV2-spike-encoding-mRNA-sequences-for-vaccines-BNT-162b2-and-mRNA-1273?

This GitHub repository, Assemblies-of-putative-SARS-CoV2-spike-encoding-mRNA-sequences-for-vaccines-BNT-162b2-and-mRNA-1273, serves as a vital resource for researchers studying RNA vaccines. It provides experimental sequence information for the RNA components of the Moderna (mRNA-1273) and Pfizer/BioNTech (BNT-162b2) COVID-19 vaccines. The project details the methodology used for obtaining and sequencing these RNAs from discarded vaccine remnants, including extraction, fragmentation, and sequencing protocols. It offers assembled contigs encoding full-length spike proteins, verifying the Pfizer sequence and providing a working assembly for Moderna's vaccine. This data is crucial for researchers and clinicians to identify therapeutic-derived RNA sequences in high-throughput RNA-seq studies, distinguishing them from host or infectious origins.

Best used for

Ideal for professors and researchers who need to analyze the RNA sequences of COVID-19 vaccines, verify reported sequence information, and identify vaccine-derived RNA in biological samples. Especially valuable for those conducting high-throughput RNA-seq studies and seeking to differentiate therapeutic RNA from host or infectious origins.

Common actions

analyze RNA sequences

verify vaccine data

research RNA vaccines

identify therapeutic RNA

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Capabilities

Key features

Moderna vaccine RNA sequence
Pfizer vaccine RNA sequence
Sequencing methodology details
Spike-encoding contig assembly
RNA stability analysis

Target Audience

professor

Integrations

Not yet documented

Pricing & Plans

Free · Open Source

Free

FAQs

What specific vaccine sequences are available in this repository?

The repository provides experimental sequence information for the RNA components of the Moderna (mRNA-1273) and Pfizer/BioNTech (BNT-162b2) COVID-19 vaccines. It includes assembled contigs encoding full-length spike proteins for both, verifying the Pfizer sequence and offering a working assembly for Moderna.

How was the RNA for sequencing obtained?

RNAs were obtained from small portions of vaccine doses that remained in vials after immunization, which would have otherwise been discarded. These remnants were then phenol-chloroform extracted using TRIzol Reagent for characterization under FDA authorization for research use.

Does the repository provide information on RNA stability?

Yes, the analysis included samples refrigerated for up to 42 days, with and without EDTA, showing that a substantial fraction of the RNA remained intact. This suggests chemical stability of the RNA itself, though not necessarily the overall vaccine formulation.

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